µP

microPublication Biology

Chiara Merigliano1ORCID logo, Maria Dilia Palumbieri2ORCID logo, Massimo Lopes2ORCID logo, and Irene Chiolo1§ORCID logo

1Molecular and Computational Biology Department, University of Southern California, Los Angeles, United States

2Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland

§Correspondence to: Irene Chiolo (chiolo@usc.edu)

Abstract

Description

Methods

Reagents

Extended Data

  • Description: Extended data script 1. This script enables automated pre-processing of focus positional data for MSD analyses. The input is an Excel file extracted from Imaris (‘Detailed’.csv), and re-ordered by track ID. In this file, the positional data for each focus are shown for subsequent time points, and followed by the next focus. The script creates separate files, one for each focus, already formatted for MSD analyses, i.e. with time points starting from 001 in the first column and XYZ coordinates in the 3 subsequent columns. The resulting set of files is entered as is a previously published script for MSD calculation (Caridi et al. 2018b).. Resource Type: Text. DOI: 10.22002/x5gxk-2xk32

Acknowledgements

Funding

Author Contributions

  • Chiara Merigliano: Investigation, Writing - original draft, Writing - review & editing
  • Maria Dilia Palumbieri: Investigation, Writing - review & editing
  • Massimo Lopes: Conceptualization, Funding acquisition, Supervision, Writing - review & editing
  • Irene Chiolo: Conceptualization, Funding acquisition, Supervision, Writing - original draft, Writing - review & editing

Reviewed By

Anonymous

History

  • Received: 6/14/2024
  • Revision Received: 7/27/2024
  • Accepted: 7/29/2024
  • Published Online: 7/31/2024
  • Indexed: 8/14/2024

References

  1. PubMed
  2. PubMed
  3. PubMed
  4. PubMed
  5. PubMed
  6. PubMed
  7. PubMed
  8. PubMed
  9. PubMed
  10. PubMed
  11. PubMed
  12. 10.1101/2023.12.07.570729
  13. PubMed
  14. PubMed
  15. PubMed
  16. PubMed
  17. PubMed
  18. PubMed
  19. PubMed
  20. PubMed
  21. PubMed
  22. PubMed
  23. PubMed
  24. PubMed
  25. PubMed
  26. PubMed
  27. PubMed
  28. PubMed
  29. PubMed

Copyright

© 2024 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

PubMed Central: 11325199

PubMed: 39149411

microPublication Biology is published by

1200 E. California Blvd. MC 1-43 Pasadena, CA 91125

The microPublication project is supported by

The National Institute of Health -- Grant #: 1U01LM012672-01

microPublication Biology:ISSN: 2578-9430